Large Perennial Fruit tree for "LEAF MINER " on Citrus (Grape Fruit in this study)

Study Investigator: J. Curtis, Phd

Location of Study: Florida, USA, open field

Study Period: Sept. to October 2018

[T2, T5, T7] Treatments for this trial with CYAN 365® in Open Field at: 1 Qt/acre Cyan 365®
App A

1 Quart/Acre

Aug.

TREATMENTS

Applied: ONE TIME (A) and trial replicated 4 times

.........................................................

Treatment 1 [T1]: Control AKA Ctrl

Applied: Replicated 4 times

  • • Untreated

.........................................................

Treatment 2 [T2]: (Cyan 365® by itself)

Applied: Only one time (A) and treatment replicated 4 times

  • • Ctrl [T1]
  • • Cyan 365® @ 1 Qt/Acre

.........................................................

Treatment 3 [T3]: Grower Std #1 AKA GS1

Applied: Only one time (A) and treatment replicated 4 times

  • • Ctrl [T1]
  • • AGRIMEK SL @ 4.25Floz/A

.........................................................

Treatment 4 [T4]: 

Applied: Only one time (A) and treatment replicated 4 times

  • • Ctrl [T1]
  • • AGRIMEK SL @ 4.25 Floz/A
  • • 435 Citrus oil (1%V/V)

.........................................................

Treatment 5 [T5]: (Cyan 365® inside)

Applied: Only one time (A) and treatment replicated 4 times

  • • Ctrl [T1]
  • • AGRIMEK SL @ 4.25 Floz/A 
  • • CYAN 365® 1Qt/Acre

 

.........................................................

Treatment 6 [T6]: 

Applied: Only one time (A) and treatment replicated 4 times

  • • Ctrl [T1]
  • • INTREPID @ 8 Floz/Acre

.........................................................

Treatment 7 [T7]: (Cyan 365® inside)

Applied: Only one time (A) and treatment replicated 4 times

  • • Ctrl [T1]
  • •  INTREPID @ 8 Floz/Acre
  • • CYAN 365® 1Qt/Acre
AGRONOMIC PROTOCOL
  • • This trial was conducted in open field with a large and reputed citrus grower within Indian river district, FL
  • • Grapefruit trees (cv. red on cleo rootstock) were planted in 1977 (40 yrs old, perennial height = 35  ft).  The row spacing was 25 ft and tree spacing was 16 ft.
  • • Each plot comprised four (4) trees (25 ft width x 64 ft length) and each plot was separated by an untreated buffer tree.  There were four (4) replicate plots for each treatment.
  • • No grower applications of INSECTICIDES were made during this study.
APPLICATIONS
  • Foliar Application (Apps. A; Trts 2-7)
  • A SINGLE FOLIAR AIRBLAST SPRAY application were made on: 
    • • 09/07/2018 (App. A)
METHODOLOGY
  • • Application was made by airblast
  • • Treatments were applied with a tractor mounted sprayer (Rears Pak-Blast, 100 gallon sprayer) at flush emergence and expansion (ca. 1/4-1 inch in length).
  • • Treatments were applied at 100 GPA (spray mix = 13.6 gallon + 6.4 gallon overage = 20 gallon spray mix), 200 PSI and at a tractor speed of 2.7 MPH (1800 RPM, Gear B2);
  • Each side of the tree in each plot was sprayed with 5 nozzles (ceramic: D8 x 5; all ceramic nozzles were fitted with a D25 whirlplate).
EVALUATIONS
  • CITRUS LEAF MINER (PHYNCI)
  • Leaf Miner were counted from 9/14/2018 to 10/12/2018.
  • In each plot, PHYNCI mines were counted on 10 separate flushes (10 BRANCH/1 PLOT).
  • All mines were examined to determine if larvae were living or dead.
PHYTO-TOXICITY
  • • Trees in each plot were examined for signs of phytotoxicity (discoloration etc.) 

 

Study Results


-82%

Reduction rate of Leaf Miner between [T6] Intre. and the Ctrl [T1] no insecticide

-84.9%

Reduction rate of Leaf Miner between [T3] Agr and the Ctrl [T1] no insecticide

-95.2%

Reduction rate of Leaf Miner between [T5] Agr+Cyan365 and the Ctrl [T1] no insecticide

+68.6%

More efficacy when Cyan 365® is added to Agrimek [T3]

+59%

More efficacy in [T5] (Cyan365+Agrimek) than in [T6] Intrepid

+35.8%

Is the degree of efficacy brought by Cyan 365® with Intrepid.

-43.8%

Reduction of the Leaf Miner in [T2] (Cyan 365 solo) Vs [T1] Ctrl with no insecticide

0%

Cyan 365® is zero Phyto-toxicity

[T5] BEST

Overall best treatment (Insecticide+Cyan365)

This study took place in the same time as for the one on "Asian Citrus Psyllid", same spot, same grower, same trees, same treatments, same weather conditions, all the same...

The purpose of this study is not to demonstrate that Cyan 365® can substitute to the insecticide but to demonstrate that when Cyan 365® is combined with it, it improves its efficacy and perform so even better. 

Thanks also to this study, we can imagine that using less chemicals for a better result is something reachable and provable.

Note: it is important to enlighten that these results have been obtained with only one application at 1Qt/acre of Cyan 365®.

Objectives: To demonstrate the ability of CYAN 365® to improve the efficacy of the insecticide made for helping to reduce the "Leaf Miner" on citrus.

For a better view of the graphs and charts, reposition your mobile device into landscape mode.

LEAF MINER ON CITRUS

Often held responsible as Canker precursor.

CITRUS LEAF MINER'S PRESENCE

This graph represent the average number of Leaf Miner counted according the 6 days count measures. Again each treatment replicated 4 times.

Results are: (By passing the cursor over the bars of the graph, result and legend will appear)

  • Average count over the course of the 6 days count:
  • Count measure dates: 9/10/18, 9/14/18, 9/21/18, 9/28/18, 10/5/18, 10/12/18, 
  • Average Data: 
  • [T1] 139.6 (Ctrl with no insecticides)
  • [T2] 78.4 (Ctrl+Cyan365 @ 1 Q/Acre+no insecticides) 
  • [T3] 21 (Agrimek SL @4.25 Floz/acre)
  • [T4] 8.4 (Agrimek SL @4.25 Floz/acre + Citrus Oil (1% V/V)
  • [T5] 6.6 (Cyan 365® 1Q/A + Agrimek SL @ 4.25 Floz/acre )
  • [T6] 25.1 (Intrepid @ 8Floz/Acre)
  • [T7] 16.1 (Intrepid @ 8Floz/Acre+Cyan 365® @ 1 Q/Acre)

-95% is the overall rate of Leaf Miner reduction between [T5] and the Control [T1]

-68,6% less Leaf Miner in [T5] (combo agrimek+Cyan365) than in [T3] (Agrimek SL) , Cyan 365® brings an improvement of the insecticide (agrimek SL) by +68.6%

-59% less Leaf Miner between [T5] (combo agrimek+Cyan365) and [T6] (Intrepid). In other word, if you add Cyan 365® to Intrepid as detailed here, you will gain 59% efficacy at helping to reduce Citrus Leaf Miner.

+35.8% is the degree of efficacy brought by Cyan 365® with Intrepid. [T6] Vs [T7]

-43.8% is what Cyan 365® is capable to do by itself in helping to reduce Leaf Miner count by comparison with the Ctrl. [T2] Vs [T1]

[T5] IS THE BEST TREATMENT

Progression / Regression of Leaf Miner
Progression / Regression of the Citrus Leaf Miner within the two months study.

This graph represent the data collected at each date of measurment (6 dates). Again all treatments replicated 4 times.

Results are: (By passing the cursor over the bars of the graph, result and legend will appear)

  • Average count over the course of the 6 days count:
  • Count measure dates: 9/10/18, 9/14/18, 9/21/18, 9/28/18, 10/5/18, 10/12/18, 
  • Treatments reminder: 
  • [T1] Ctrl with no insecticides
  • [T2] Ctrl+Cyan365 @ 1 Q/Acre+no insecticides
  • [T3] Agrimek SL @4.25 Floz/acre
  • [T4] Agrimek SL @4.25 Floz/acre + Citrus Oil (1% V/V)
  • [T5] Cyan 365® 1Q/A + Agrimek SL @ 4.25 Floz/acre
  • [T6] Intrepid @ 8Floz/Acre
  • [T7] Intrepid @ 8Floz/Acre+Cyan 365® @ 1 Q/Acre

It is quite evident with these curbs where Cyan 365® helps to improve the efficacy of the insecticide.

9/28/18 and 10/12/18 are the most significant dates to compare.

[T5] IS THE BEST TREATMENT