Large Perennial Fruit tree for "ASIAN CITRUS PSYLLID " on Citrus (Grape Fruit in this study)

Study Investigator: J. Curtis, Phd

Location of Study: Florida, USA, open field

Study Period: Sept. to October 2018

[T2, T5, T7] Treatments for this trial with CYAN 365® in Open Field at: 1 Qt/acre Cyan 365®
App A

1 Quart/Acre

Aug.

TREATMENTS

Applied: ONE TIME (A) and trial replicated 4 times

.........................................................

Treatment 1 [T1]: Control AKA Ctrl

Applied: Replicated 4 times

  • • Untreated

.........................................................

Treatment 2 [T2]: (Cyan 365® by itself)

Applied: Only one time (A) and treatment replicated 4 times

  • • Ctrl [T1]
  • • Cyan 365® @ 1 Qt/Acre

.........................................................

Treatment 3 [T3]: Grower Std #1 AKA GS1

Applied: Only one time (A) and treatment replicated 4 times

  • • Ctrl [T1]
  • • AGRIMEK SL @ 4.25Floz/A

.........................................................

Treatment 4 [T4]: 

Applied: Only one time (A) and treatment replicated 4 times

  • • Ctrl [T1]
  • • AGRIMEK SL @ 4.25 Floz/A
  • • 435 Citrus oil (1%V/V)

.........................................................

Treatment 5 [T5]: (Cyan 365® inside)

Applied: Only one time (A) and treatment replicated 4 times

  • • Ctrl [T1]
  • • AGRIMEK SL @ 4.25 Floz/A 
  • • CYAN 365® 1Qt/Acre

 

.........................................................

Treatment 6 [T6]: 

Applied: Only one time (A) and treatment replicated 4 times

  • • Ctrl [T1]
  • • INTREPID @ 8 Floz/Acre

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Treatment 7 [T7]: (Cyan 365® inside)

Applied: Only one time (A) and treatment replicated 4 times

  • • Ctrl [T1]
  • •  INTREPID @ 8 Floz/Acre
  • • CYAN 365® 1Qt/Acre
AGRONOMIC PROTOCOL
  • • This trial was conducted in open field with a large and reputed citrus grower within Indian river district, FL
  • • Grapefruit trees (cv. red on cleo rootstock) were planted in 1977 (40 yrs old, perennial height = 35  ft).  The row spacing was 25 ft and tree spacing was 16 ft.
  • • Each plot comprised four (4) trees (25 ft width x 64 ft length) and each plot was separated by an untreated buffer tree.  There were four (4) replicate plots for each treatment.
  • • No grower applications of INSECTICIDES were made during this study.
APPLICATIONS
  • Foliar Application (Apps. A; Trts 2-7)
  • A SINGLE FOLIAR AIRBLAST SPRAY application were made on: 
    • • 09/07/2018 (App. A)
METHODOLOGY
  • • Application was made by airblast
  • • Treatments were applied with a tractor mounted sprayer (Rears Pak-Blast, 100 gallon sprayer) at flush emergence and expansion (ca. 1/4-1 inch in length).
  • • Treatments were applied at 100 GPA (spray mix = 13.6 gallon + 6.4 gallon overage = 20 gallon spray mix), 200 PSI and at a tractor speed of 2.7 MPH (1800 RPM, Gear B2);
  • Each side of the tree in each plot was sprayed with 5 nozzles (ceramic: D8 x 5; all ceramic nozzles were fitted with a D25 whirlplate).
EVALUATIONS
  • ASIAN CITRUS PSYLLID (ACP) or (DIAACI)
  • Psyllids  were counted on 9/10/2018, 9/14/2018, 9/21/2018, 9/28/2018, 10/5/2018 and 10/12/2018.
  • Adults:
    • • A sweep net was used to capture adults on each of two trees in the center of each plot (2 SWEEP/1 PLOT).  Each tree was sweeped for 10 seconds and the contents of the net counted.
  • Eggs & Nymphs:
    • • In each plot, eggs and nymphs were counted with a hand lens on 10 separate flushes (10 BRANCH/1 PLOT).
PHYTO-TOXICITY
  • • Trees in each plot were examined for signs of phytotoxicity (discoloration etc.) 

 

Study Results


-60%

Reduction rate of ADULTS' ACP between [T5] Agr+Cyan365 and the Ctrl [T1] no insecticide

-77.3%

Reduction rate of EGGS' ACP between [T5] Agr+Cyan365 and the Ctrl [T1] no insecticide

-81.8%

Reduction rate of NYMPHS' ACP between [T5] Agr+Cyan365 and the Ctrl [T1] no insecticide

-22%

Reduction rate of ADULTS' ACP between [T2] Cyan365 ALONE and the Ctrl [T1] no insecticide

-57.5%

Reduction rate of EGGS' ACP between [T2] Cyan365 ALONE and the Ctrl [T1] no insecticide

-61%

Reduction rate of NYMPHS' ACP between [T2] Cyan365 ALONE and the Ctrl [T1] no insecticide

0%

Cyan 365® is zero Phyto-toxicity

[T5] BEST

Overall best treatment (Insecticide+Cyan365)

This study here is not to demonstrate that Cyan 365® is curing any disease and certainly not the HLB. Cyan 365® is not an insecticide and there is no such claims in this study.

The primary matter in this study is to demonstrate that Cyan 365® conjonctly used with an insecticide make the program performing even better. As the matter of facts, it is about to reduce and combat the proliferation of the ACP's adults, eggs and nymphs when Cyan 365® is combined with an insecticide.

On a second level, we can imagine that using less chemicals for a better result is something reachable and provable.

Also it is very interesting to note and deserves to be enhanced: The results obtained in this agronomic experiment have been achieved with ONLY one application but replicated 4 times.

 

Objectives: To demonstrate the ability of CYAN 365® to improve the efficacy of the insecticide used by the citrus growers to help at reducing the population of the ASIAN CITRUS PSYLLID (responsible of the degenerative disease also called "Greening and/or "HLB) on Citrus.

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ACP (Asian Citrus Psyllid) ADULTS
Average of ACP's ADULTS count

This graph represent the average number of ADULT's ACP counted according the 6 days count measures. Again replicated 4 times.

Results are: (By passing the cursor over the bars of the graph, result and legend will appear)

  • Average count over the course of the 6 days count:
  • Count measure dates: 9/10/18, 9/14/18, 9/21/18, 9/28/18, 10/5/18, 10/12/18, 
  • Average Data: 
  • [T1] 42.6 (Ctrl with no insecticides)
  • [T2] 33.3 (Ctrl+Cyan365® @ 1 Q/Acre+no insecticides) 
  • [T3] 20.4 (Agrimek SL @4.25 Floz/acre)
  • [T4] 20.2 (Agrimek SL @4.25 Floz/acre + Citrus Oil (1% V/V)
  • [T5] 17 (Cyan 365® 1Q/A + Agrimek SL @ 4.25 Floz/acre )
  • [T6] 25 (Intrepid @ 8Floz/Acre)
  • [T7] 21.8 (Intrepid @ 8Floz/Acre+Cyan 365® @ 1 Q/Acre)

-60% is the overall rate of Adult ACP's reduction between [T5] and the Control [T1]

-16.6% Less ACP's adults count between [T5] (combo agrimek+Cyan365) and [T3] (Agrimek SL) , Cyan 365® brings an improvement of the insecticide of about +16.6%

-32% count is the difference between [T5] (combo agrimek+Cyan365) and [T6] (Intrepid)

+13% is the degree of efficacy brought by Cyan 365® with Intrepid. [T6] Vs [T7]

-22% is what Cyan 365® is capable to do by itself in helping to reduce ACP's count by comparison with the Ctrl. [T2] Vs [T1]

[T5] IS THE BEST TREATMENT

ACP'S EGGS
Average of ACP's EGGS

This graph represent the average number of ACP's EGGS counted according the 6 days count measures. Again replicated 4 times.

Results are: (By passing the cursor over the bars of the graph, result and legend will appear)

  • Average count over the course of the 6 days count:
  • Count measure dates: 9/10/18, 9/14/18, 9/21/18, 9/28/18, 10/5/18, 10/12/18, 
  • Average Data: 
  • [T1] 821.2 (Ctrl with no insecticides)
  • [T2] 348.9 (Ctrl+Cyan365® @ 1 Q/Acre+no insecticides) 
  • [T3] 217.7 (Agrimek SL @4.25 Floz/acre)
  • [T4] 259.7 (Agrimek SL @4.25 Floz/acre + Citrus Oil (1% V/V)
  • [T5] 185.9 (Cyan 365® 1Q/A + Agrimek SL @ 4.25 Floz/acre )
  • [T6] 279.4 (Intrepid @ 8Floz/Acre)
  • [T7] 265.3 (Intrepid @ 8Floz/Acre+Cyan 365® @ 1 Q/Acre)

-77.3% Less ACP's EGGS in [T5] than in the Control [T1]

-28.4% Less ACP's eggs in [T5] (combo agrimek+Cyan365) than in [T4] (combo Agrimek+citrus oil) , Cyan 365® brings an improvement of the insecticide of about +28.4%

-33.5% count is the difference between [T5] (combo agrimek+Cyan365®) and [T6] (Intrepid)

-57.5% is what Cyan 365® is capable to do by itself in helping to reduce ACP's EGGS count by comparison with the Ctrl. [T2] Vs [T1]

[T5] IS THE BEST TREATMENT

ACP'S NYMPHS
Average of ACP's NYMPHS

This graph represent the average number of ACP's NYMPHS counted according the 6 days count measures. Again replicated 4 times.

Results are: (By passing the cursor over the bars of the graph, result and legend will appear)

  • Average count over the course of the 6 days count:
  • Count measure dates: 9/10/18, 9/14/18, 9/21/18, 9/28/18, 10/5/18, 10/12/18, 
  • Average Data: 
  • [T1] 549 (Ctrl with no insecticides)
  • [T2] 213.4 (Ctrl+Cyan365® @ 1 Q/Acre+no insecticides) 
  • [T3] 97.2 (Agrimek SL @4.25 Floz/acre)
  • [T4] 104.2 (Agrimek SL @4.25 Floz/acre + Citrus Oil (1% V/V)
  • [T5] 99.6 (Cyan 365® 1Q/A + Agrimek SL @ 4.25 Floz/acre )
  • [T6] 153.9 (Intrepid @ 8Floz/Acre)
  • [T7] 133.4 (Intrepid @ 8Floz/Acre+Cyan 365® @ 1 Q/Acre)

-81.8% Less overall ACP's NYMPHS between [T5] and the Control [T1]

-35.2% Less ACP's Nymphs in [T5] (combo agrimek+Cyan365®) than in [T6] (Intrepid)

-61% is what Cyan 365® is capable to do by itself in helping to reduce ACP's NYMPHS count by comparison with the Ctrl. [T2] Vs [T1]

[T5] IS THE BEST TREATMENT with [T3]

OVERALL ACP 's COUNT (adult, egg, nymph)
  • [T5] is the best overall Treatment to reduce the population of ACP's (adults, eggs, nymphs)
  • Accordingly, the association of the insecticide (Agrimek SL) with Cyan 365® is beneficiary for the Grower to combat with an improved efficacy the Asian Citrus Psyllid.